Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Bioworld PEB- Protein Extraction Buffer (5x), 250 mL
Tris Protein Extraction Buffer (PEB), 5X Lysis buffer for extracting proteins from cells. Technical Information:5X concentrationTris-HCL, pH 8.0 (0.5M)B-mercaptoethanol (100mM)EDTA (20mM)Add protease inhibitor according to your protocol
Electron Microscopy Sciences Tris Maleate Buffer 0.2M, pH range 6.4-8.0 250 ML
Please specify pH when ordering.
pH: 6.4-8.0
Biotang Inc TRIS-HCL Buffer 1.0M, pH 7.5 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 7.5 (0.2 micron filtered), 500 ml.
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.5 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.5 (0.2 micron filtered), 500 ml.
Teknova 10X TBE DNA Sequencing Grade Solution. 1000mL, Non-Sterile 2 pack.
890mM Tris,890mM Boric Acid, 20mM Ethylenediaminetetraacetic acid (EDTA)
Alkali Scientific TRIS Glycine SDS Buffer, 10X Powder, 184 Grams of Powder, Makes 1 Liter of Solution
TRIS Glycine SDS Buffer (10X Powder) is a commonly used buffer solution in electrophoresis and protein separation techniques. This 184g powder is designed to make 1 liter of 10X concentrated solution, ideal for preparing protein gels for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis). The buffer helps maintain a consistent pH and conductivity during protein separation, ensuring reproducible results. It is widely used in laboratories for protein analysis, molecular biology applications, and biochemistry experiments. TRIS Glycine SDS Buffer is essential for the proper functioning of electrophoresis systems, helping researchers isolate proteins based on size and charge in a reliable and efficient manner.
Sigma Aldrich Fine Chemicals Biosciences BIS-TRIS Propane | 64431-96-5 | MFCD00004689 | 100g
BIS-TRIS Propane | Purity: 99% | Mol Wt: 282.33 | 64431-96-5 | MFCD00004689 | 100g
Hampton Research HR2-795/1.0 M BIS-TRIS propane pH 7.0 - 100 ml
HR2-795/1.0 M BIS-TRIS propane pH 7.0 - 100 ml. Please note items from this supplier are non-returnable.
Bioworld TBE Buffer, Quick-Mix Pack (25X), 2 PK(s)
Product DescriptionTBE (Tris-Borate-EDTA) quick-mix pack prepares 1L of 25X concentrate. Each pack contains 0.089M Tris, 0.089M Borate, and 0.002M EDTA. Tris solutions are effective buffers for slightly basic conditions. Borate, a derivative of boric acid, is a weak proton donor but a Lewis acid that can accept an electron pair. EDTA is a chelator of divalent cations, mainly Mg2+. TBE is most commonly used for nucleic acid electrophoresis.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Quality Biological Inc Tris-HCl (2M) pH 8.8, 500 mL
Tris HCl Buffer is compatible with many enzymes in molecular biology (e.g. DNA modifying enzymes). Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959).
Applications: Tris HCl (2M), pH 8.8 is useful with SDS-PAGE and Electrophoresis.
Storage Conditions: 15 °C to 30 °C
Shipping Condition: ambient temperature
Sterile Filtered
DNase RNase and Protease Tested
Max Shelf Life: 36 months from date of manufacture
Product and/or packaging contains recycled/renewable content. Product and/or packaging is recyclable or reduces waste. Product and/or packaging is safer to human and environmental health.